Detailed information of TA system TA228715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3708930..3709604
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: LVJ67_RS17560
• Protein ID: WP_003417282.1
• Coordinates: 3708930..3709358 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: LVJ67_RS17565
• Protein ID: WP_003417286.1
• Coordinates: 3709362..3709604 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS17535 (LVJ67_17535)	3704752..3705153	-	402	WP_003417264.1	cytidine deaminase	-
LVJ67_RS17540 (LVJ67_17540)	3705390..3705728	+	339	WP_003417267.1	succinate dehydrogenase, cytochrome b556 subunit	-
LVJ67_RS17545 (LVJ67_17545)	3705725..3706159	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
LVJ67_RS17550 (LVJ67_17550)	3706288..3708060	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
LVJ67_RS17555 (LVJ67_17555)	3708060..3708851	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
LVJ67_RS17560 (LVJ67_17560)	3708930..3709358	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ67_RS17565 (LVJ67_17565)	3709362..3709604	-	243	WP_003417286.1	CopG family transcriptional regulator	Antitoxin
LVJ67_RS17570 (LVJ67_17570)	3709726..3710340	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
LVJ67_RS17575 (LVJ67_17575)	3710337..3711002	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
LVJ67_RS17580 (LVJ67_17580)	3711003..3711536	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
LVJ67_RS17585 (LVJ67_17585)	3711533..3711907	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
LVJ67_RS17595 (LVJ67_17595)	3713362..3714498	-	1137	WP_003417297.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T228715 WP_003417282.1 NZ_CP089772:c3709358-3708930 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T228715 NZ_CP124338:c133843-133538 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT228715 WP_003417286.1 NZ_CP089772:c3709604-3709362 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT228715 NZ_CP124338:c134063-133845 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59