Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3184379..3185066 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag LVJ67_RS15170 Protein ID WP_003414624.1
Coordinates 3184623..3185066 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag LVJ67_RS15165 Protein ID WP_003414620.1
Coordinates 3184379..3184636 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS15145 (LVJ67_15140) 3179699..3180553 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
LVJ67_RS15150 (LVJ67_15145) 3180609..3181772 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
LVJ67_RS15155 (LVJ67_15150) 3181789..3183003 - 1215 WP_003899518.1 zinc metalloprotease Rip -
LVJ67_RS15160 (LVJ67_15155) 3183011..3184252 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
LVJ67_RS15165 (LVJ67_15160) 3184379..3184636 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
LVJ67_RS15170 (LVJ67_15165) 3184623..3185066 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ67_RS15175 (LVJ67_15170) 3185146..3185808 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
LVJ67_RS15180 (LVJ67_15175) 3185904..3186095 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
LVJ67_RS15185 (LVJ67_15180) 3186427..3188175 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
LVJ67_RS15190 (LVJ67_15185) 3188271..3188852 + 582 WP_003414644.1 fasciclin domain-containing protein -
LVJ67_RS15195 (LVJ67_15190) 3188952..3189218 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T228714 WP_003414624.1 NZ_CP089772:3184623-3185066 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T228714 NZ_CP124337:c4531687-4531514 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT228714 WP_003414620.1 NZ_CP089772:3184379-3184636 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT228714 NZ_CP124337:c4532011-4531715 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References