Detailed information of TA system TA228714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3184379..3185066
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: LVJ67_RS15170
• Protein ID: WP_003414624.1
• Coordinates: 3184623..3185066 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: LVJ67_RS15165
• Protein ID: WP_003414620.1
• Coordinates: 3184379..3184636 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS15145 (LVJ67_15140)	3179699..3180553	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
LVJ67_RS15150 (LVJ67_15145)	3180609..3181772	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
LVJ67_RS15155 (LVJ67_15150)	3181789..3183003	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
LVJ67_RS15160 (LVJ67_15155)	3183011..3184252	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
LVJ67_RS15165 (LVJ67_15160)	3184379..3184636	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
LVJ67_RS15170 (LVJ67_15165)	3184623..3185066	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ67_RS15175 (LVJ67_15170)	3185146..3185808	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
LVJ67_RS15180 (LVJ67_15175)	3185904..3186095	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
LVJ67_RS15185 (LVJ67_15180)	3186427..3188175	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
LVJ67_RS15190 (LVJ67_15185)	3188271..3188852	+	582	WP_003414644.1	fasciclin domain-containing protein	-
LVJ67_RS15195 (LVJ67_15190)	3188952..3189218	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T228714 WP_003414624.1 NZ_CP089772:3184623-3185066 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T228714 NZ_CP124337:c4531687-4531514 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT228714 WP_003414620.1 NZ_CP089772:3184379-3184636 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT228714 NZ_CP124337:c4532011-4531715 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0