Detailed information of TA system TA228713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3178778..3179326
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: LVJ67_RS15140
• Protein ID: WP_003414602.1
• Coordinates: 3179063..3179326 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: LVJ67_RS15135
• Protein ID: WP_003414599.1
• Coordinates: 3178778..3179059 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS15110 (LVJ67_15105)	3174401..3175258	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
LVJ67_RS15115 (LVJ67_15110)	3175300..3175884	-	585	WP_003899514.1	DUF1707 domain-containing protein	-
LVJ67_RS15120 (LVJ67_15115)	3175988..3176236	+	249	WP_003913411.1	antitoxin VapB23	-
LVJ67_RS15125 (LVJ67_15120)	3176233..3176613	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
LVJ67_RS15130 (LVJ67_15125)	3176695..3178506	-	1812	WP_003906921.1	penicillin-binding protein	-
LVJ67_RS15135 (LVJ67_15130)	3178778..3179059	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
LVJ67_RS15140 (LVJ67_15135)	3179063..3179326	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ67_RS15145 (LVJ67_15140)	3179699..3180553	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
LVJ67_RS15150 (LVJ67_15145)	3180609..3181772	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
LVJ67_RS15155 (LVJ67_15150)	3181789..3183003	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
LVJ67_RS15160 (LVJ67_15155)	3183011..3184252	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T228713 WP_003414602.1 NZ_CP089772:3179063-3179326 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T228713 NZ_CP124337:4519913-4520290 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACCCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT228713 WP_003414599.1 NZ_CP089772:3178778-3179059 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT228713 NZ_CP124337:4519455-4519823 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGCTG
TGGTTGCGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8