Detailed information of TA system TA228710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3110369..3110939
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: LVJ67_RS14790
• Protein ID: WP_003414166.1
• Coordinates: 3110369..3110725 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: LVJ67_RS14795
• Protein ID: WP_003901465.1
• Coordinates: 3110709..3110939 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS14770 (LVJ67_14770)	3105821..3107509	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
LVJ67_RS14775 (LVJ67_14775)	3107513..3107839	-	327	WP_003414157.1	hypothetical protein	-
LVJ67_RS14780 (LVJ67_14780)	3108012..3108599	+	588	WP_003914429.1	DUF3558 family protein	-
LVJ67_RS14785 (LVJ67_14785)	3108618..3110267	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
LVJ67_RS14790 (LVJ67_14790)	3110369..3110725	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
LVJ67_RS14795 (LVJ67_14795)	3110709..3110939	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
LVJ67_RS14800 (LVJ67_14800)	3110982..3112025	-	1044	WP_031697622.1	DUF2293 domain-containing protein	-
LVJ67_RS14805 (LVJ67_14805)	3112024..3112491	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
LVJ67_RS14810 (LVJ67_14810)	3112667..3112921	-	255	WP_003917684.1	hypothetical protein	-
LVJ67_RS14815 (LVJ67_14815)	3113069..3113473	+	405	WP_009938577.1	hypothetical protein	-
LVJ67_RS14820 (LVJ67_14820)	3113470..3113661	+	192	WP_003414184.1	hypothetical protein	-
LVJ67_RS14825 (LVJ67_14825)	3113878..3114138	+	261	Protein_2926	transposase	-
LVJ67_RS14830 (LVJ67_14830)	3115248..3115505	+	258	WP_003899489.1	hypothetical protein	-
LVJ67_RS14835 (LVJ67_14835)	3115610..3115921	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T228710 WP_003414166.1 NZ_CP089772:c3110725-3110369 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T228710 NZ_CP124337:c4027899-4027585 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT228710 WP_003901465.1 NZ_CP089772:c3110939-3110709 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT228710 NZ_CP124337:c4028135-4027902 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3