Detailed information of TA system TA228706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2931473..2932187
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: LVJ67_RS13740
• Protein ID: WP_003413460.1
• Coordinates: 2931747..2932187 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: LVJ67_RS13735
• Protein ID: WP_003413456.1
• Coordinates: 2931473..2931760 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS13700 (LVJ67_13695)	2926895..2927140	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ67_RS13705 (LVJ67_13700)	2927137..2927541	+	405	WP_003899389.1	type II toxin-antitoxin system VapC family toxin	-
LVJ67_RS13710 (LVJ67_13705)	2927758..2928378	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
LVJ67_RS13715 (LVJ67_13710)	2928389..2928883	+	495	WP_003413444.1	DUF2617 family protein	-
LVJ67_RS13720 (LVJ67_13715)	2928880..2929311	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
LVJ67_RS13725 (LVJ67_13720)	2929336..2929794	+	459	WP_003413451.1	DUF350 domain-containing protein	-
LVJ67_RS13730 (LVJ67_13725)	2929791..2931362	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
LVJ67_RS13735 (LVJ67_13730)	2931473..2931760	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
LVJ67_RS13740 (LVJ67_13735)	2931747..2932187	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ67_RS13745 (LVJ67_13740)	2932208..2932963	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
LVJ67_RS13750 (LVJ67_13745)	2933096..2933692	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
LVJ67_RS13755 (LVJ67_13750)	2933700..2934545	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
LVJ67_RS13760 (LVJ67_13755)	2934574..2935473	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
LVJ67_RS13765 (LVJ67_13760)	2935601..2936275	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T228706 WP_003413460.1 NZ_CP089772:2931747-2932187 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T228706 NZ_CP124337:2946931-2947209 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT228706 WP_003413456.1 NZ_CP089772:2931473-2931760 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT228706 NZ_CP124337:2946738-2946929 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8