Detailed information of TA system TA228703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2869558..2870267
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ26
• Locus tag: LVJ67_RS13455
• Protein ID: WP_003413164.1
• Coordinates: 2869558..2869971 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: LVJ67_RS13460
• Protein ID: WP_003413167.1
• Coordinates: 2870010..2870267 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS13425 (LVJ67_13420)	2864611..2865816	-	1206	WP_003413027.1	chorismate synthase	-
LVJ67_RS13430 (LVJ67_13425)	2865924..2866238	+	315	WP_009937839.1	hypothetical protein	-
LVJ67_RS13435 (LVJ67_13430)	2866534..2867745	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
LVJ67_RS13440 (LVJ67_13435)	2867872..2868531	+	660	WP_003900846.1	LppA family lipoprotein	-
LVJ67_RS13445 (LVJ67_13440)	2868528..2869190	+	663	WP_003900848.1	LppA family lipoprotein	-
LVJ67_RS13450 (LVJ67_13445)	2869187..2869465	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ67_RS13455 (LVJ67_13450)	2869558..2869971	+	414	WP_003413164.1	PIN domain nuclease	Toxin
LVJ67_RS13460 (LVJ67_13455)	2870010..2870267	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
LVJ67_RS13465 (LVJ67_13460)	2870264..2870641	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
LVJ67_RS13470 (LVJ67_13465)	2870657..2871031	-	375	WP_003413177.1	hypothetical protein	-
LVJ67_RS13475 (LVJ67_13470)	2871131..2871526	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
LVJ67_RS13480 (LVJ67_13475)	2871523..2871768	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
LVJ67_RS13485 (LVJ67_13480)	2872179..2872598	-	420	WP_003413190.1	A24 family peptidase	-
LVJ67_RS13490 (LVJ67_13485)	2872610..2873419	-	810	WP_003413193.1	shikimate dehydrogenase	-
LVJ67_RS13495 (LVJ67_13490)	2873416..2874669	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
LVJ67_RS13500 (LVJ67_13495)	2874662..2875174	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15045.00 Da
• Isoelectric Point: 5.4673

>T228703 WP_003413164.1 NZ_CP089772:2869558-2869971 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T228703 NZ_CP124337:2248110-2248212 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT228703 WP_003413167.1 NZ_CP089772:2870010-2870267 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT228703 NZ_CP124337:c2248250-2247982 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: G0TQ26

Antitoxin

• AlphaFold DB: A0A829C9W5