Detailed information of TA system TA228702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2855671..2856311
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: LVJ67_RS13370
• Protein ID: WP_003412970.1
• Coordinates: 2855671..2856090 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: LVJ67_RS13375
• Protein ID: WP_003412975.1
• Coordinates: 2856087..2856311 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS13340 (LVJ67_13335)	2851256..2851978	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
LVJ67_RS13345 (LVJ67_13340)	2852495..2852722	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ67_RS13350 (LVJ67_13345)	2852719..2853120	+	402	WP_003412963.1	PIN domain-containing protein	-
LVJ67_RS13355 (LVJ67_13350)	2853155..2854075	-	921	WP_003412965.1	restriction endonuclease	-
LVJ67_RS13360 (LVJ67_13355)	2854416..2854661	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ67_RS13365 (LVJ67_13360)	2854720..2855670	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
LVJ67_RS13370 (LVJ67_13365)	2855671..2856090	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ67_RS13375 (LVJ67_13370)	2856087..2856311	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
LVJ67_RS13380 (LVJ67_13375)	2856342..2859185	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
LVJ67_RS13385 (LVJ67_13380)	2859257..2859658	-	402	WP_003412981.1	hypothetical protein	-
LVJ67_RS13390 (LVJ67_13385)	2859658..2860128	-	471	WP_003899364.1	transcription antitermination factor NusB	-
LVJ67_RS13395 (LVJ67_13390)	2860131..2860694	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T228702 WP_003412970.1 NZ_CP089772:c2856090-2855671 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T228702 NZ_CP124337:c2001978-2001604 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT228702 WP_003412975.1 NZ_CP089772:c2856311-2856087 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT228702 NZ_CP124337:c2002435-2002067 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09