Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2809487..2810139 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag LVJ67_RS13175 Protein ID WP_003412752.1
Coordinates 2809714..2810139 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag LVJ67_RS13170 Protein ID WP_003412749.1
Coordinates 2809487..2809708 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS13155 (LVJ67_13150) 2807727..2807933 - 207 WP_003899347.1 hypothetical protein -
LVJ67_RS13160 (LVJ67_13155) 2808069..2808692 + 624 WP_003900858.1 TIGR00725 family protein -
LVJ67_RS13165 (LVJ67_13160) 2808682..2809434 + 753 WP_003900859.1 hypothetical protein -
LVJ67_RS13170 (LVJ67_13165) 2809487..2809708 + 222 WP_003412749.1 antitoxin Antitoxin
LVJ67_RS13175 (LVJ67_13170) 2809714..2810139 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ67_RS13180 (LVJ67_13175) 2810162..2811343 - 1182 WP_003899351.1 dihydrolipoamide acetyltransferase family protein -
LVJ67_RS13185 (LVJ67_13180) 2811340..2812386 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
LVJ67_RS13190 (LVJ67_13185) 2812397..2813500 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
LVJ67_RS13195 (LVJ67_13190) 2813759..2814580 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
LVJ67_RS13200 (LVJ67_13195) 2814577..2815134 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T228701 WP_003412752.1 NZ_CP089772:2809714-2810139 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T228701 NZ_CP124337:1988942-1989196 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT228701 WP_003412749.1 NZ_CP089772:2809487-2809708 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT228701 NZ_CP124337:1988694-1988945 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References