Detailed information of TA system TA228700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2406931..2407460
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: LVJ67_RS11270
• Protein ID: WP_003411124.1
• Coordinates: 2406931..2407248 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: LVJ67_RS11275
• Protein ID: WP_003411127.1
• Coordinates: 2407245..2407460 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS11240 (LVJ67_11245)	2402126..2403202	+	1077	WP_003411110.1	hypothetical protein	-
LVJ67_RS11245 (LVJ67_11250)	2403199..2403480	+	282	WP_003411112.1	DUF5703 family protein	-
LVJ67_RS11250 (LVJ67_11255)	2403516..2404589	+	1074	WP_003411116.1	quinone-dependent dihydroorotate dehydrogenase	-
LVJ67_RS11255 (LVJ67_11260)	2404594..2405124	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
LVJ67_RS11260 (LVJ67_11265)	2405172..2406518	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
LVJ67_RS11270 (LVJ67_11275)	2406931..2407248	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ67_RS11275 (LVJ67_11280)	2407245..2407460	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
LVJ67_RS11280 (LVJ67_11285)	2407715..2408773	+	1059	WP_003411129.1	phosphoribosyltransferase family protein	-
LVJ67_RS11285 (LVJ67_11290)	2408903..2409259	-	357	WP_003411130.1	hypothetical protein	-
LVJ67_RS11290 (LVJ67_11295)	2409354..2410136	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
LVJ67_RS11295 (LVJ67_11300)	2410404..2410694	-	291	WP_003900476.1	YggT family protein	-
LVJ67_RS11300 (LVJ67_11305)	2410856..2411512	-	657	WP_003411133.1	cell division protein SepF	-
LVJ67_RS11305 (LVJ67_11310)	2411578..2412354	-	777	WP_003411137.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T228700 WP_003411124.1 NZ_CP089772:c2407248-2406931 [Mycobacterium tuberculosis]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T228700 NZ_CP124337:1988694-1988945 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT228700 WP_003411127.1 NZ_CP089772:c2407460-2407245 [Mycobacterium tuberculosis]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT228700 NZ_CP124337:1988942-1989196 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4