Detailed information of TA system TA228696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2259104..2259730
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: LVJ67_RS10575
• Protein ID: WP_003410075.1
• Coordinates: 2259332..2259730 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: LVJ67_RS10570
• Protein ID: WP_003911750.1
• Coordinates: 2259104..2259331 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS10560 (LVJ67_10565)	2257143..2257487	-	345	WP_003410065.1	ferredoxin family protein	-
LVJ67_RS10565 (LVJ67_10570)	2257676..2258845	-	1170	WP_003899126.1	ATP-binding protein	-
LVJ67_RS10570 (LVJ67_10575)	2259104..2259331	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ67_RS10575 (LVJ67_10580)	2259332..2259730	+	399	WP_003410075.1	PIN domain nuclease	Toxin
LVJ67_RS10580 (LVJ67_10585)	2259913..2260344	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
LVJ67_RS10585 (LVJ67_10590)	2260445..2260879	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
LVJ67_RS10590 (LVJ67_10595)	2261316..2261495	-	180	Protein_2093	hypothetical protein	-
LVJ67_RS10595 (LVJ67_10600)	2261583..2262203	+	621	WP_003410086.1	IS110 family transposase	-
LVJ67_RS10600 (LVJ67_10605)	2262157..2262747	+	591	WP_003899131.1	IS110 family transposase	-
LVJ67_RS10605 (LVJ67_10610)	2262875..2264131	-	1257	WP_003410095.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T228696 WP_003410075.1 NZ_CP089772:2259332-2259730 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T228696 NZ_CP124337:c605509-605045 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT228696 WP_003911750.1 NZ_CP089772:2259104-2259331 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT228696 NZ_CP124337:c605844-605509 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8