Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2233798..2234696 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag LVJ67_RS10450 Protein ID WP_003410001.1
Coordinates 2233798..2234358 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag LVJ67_RS10455 Protein ID WP_003410003.1
Coordinates 2234355..2234696 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS10420 (LVJ67_10425) 2228967..2229620 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ67_RS10425 (LVJ67_10430) 2229735..2229965 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ67_RS10430 (LVJ67_10435) 2230050..2230961 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ67_RS10435 (LVJ67_10440) 2231070..2231669 + 600 WP_003409989.1 L-lysine exporter -
LVJ67_RS10440 (LVJ67_10445) 2232085..2232513 + 429 WP_003906713.1 cellulose-binding protein -
LVJ67_RS10445 (LVJ67_10450) 2232739..2233278 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ67_RS10450 (LVJ67_10455) 2233798..2234358 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
LVJ67_RS10455 (LVJ67_10460) 2234355..2234696 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
LVJ67_RS10460 (LVJ67_10465) 2234782..2235039 + 258 WP_003410006.1 hypothetical protein -
LVJ67_RS10465 (LVJ67_10470) 2234940..2235275 - 336 WP_003410009.1 dehydrogenase -
LVJ67_RS10470 (LVJ67_10475) 2235364..2235708 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
LVJ67_RS10475 (LVJ67_10480) 2235702..2235950 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
LVJ67_RS10480 (LVJ67_10485) 2236050..2238365 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ67_RS10485 (LVJ67_10490) 2238362..2238634 - 273 WP_003410017.1 DUF1490 family protein -
LVJ67_RS10490 (LVJ67_10495) 2238687..2239043 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T228694 WP_003410001.1 NZ_CP089772:c2234358-2233798 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T228694 NZ_CP124337:188541-188648 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGTTCCGGTCATTACTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT228694 WP_003410003.1 NZ_CP089772:c2234696-2234355 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT228694 NZ_CP124337:c188484-188427 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References