Detailed information of TA system TA228690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2195702..2196405
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: LVJ67_RS10195
• Protein ID: WP_003409778.1
• Coordinates: 2195702..2196031 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: LVJ67_RS10200
• Protein ID: WP_003409780.1
• Coordinates: 2196028..2196405 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS10175 (LVJ67_10180)	2192085..2193155	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
LVJ67_RS10180 (LVJ67_10185)	2193152..2193667	+	516	WP_003409718.1	flavin reductase family protein	-
LVJ67_RS10185 (LVJ67_10190)	2193664..2194725	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LVJ67_RS10190 (LVJ67_10195)	2194722..2195492	+	771	WP_003409775.1	SDR family oxidoreductase	-
LVJ67_RS10195 (LVJ67_10200)	2195702..2196031	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LVJ67_RS10200 (LVJ67_10205)	2196028..2196405	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
LVJ67_RS10205 (LVJ67_10210)	2196402..2196992	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
LVJ67_RS10210 (LVJ67_10215)	2197047..2198411	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
LVJ67_RS10215 (LVJ67_10220)	2198566..2199018	-	453	WP_003899095.1	lipoprotein	-
LVJ67_RS10220 (LVJ67_10225)	2199082..2199483	+	402	WP_003409869.1	hypothetical protein	-
LVJ67_RS10225 (LVJ67_10230)	2199476..2199658	-	183	WP_003409870.1	hypothetical protein	-
LVJ67_RS10230 (LVJ67_10235)	2199772..2200122	-	351	WP_003899096.1	hypothetical protein	-
LVJ67_RS10235 (LVJ67_10240)	2200133..2201035	-	903	WP_003899097.1	hypothetical protein	-
LVJ67_RS10240 (LVJ67_10245)	2201056..2201247	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T228690 WP_003409778.1 NZ_CP089772:c2196031-2195702 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T228690 NZ_CP124334:4957229-4957483 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT228690 WP_003409780.1 NZ_CP089772:c2196405-2196028 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT228690 NZ_CP124334:4956981-4957232 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8