Detailed information of TA system TA022869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-AbkA/BrnT_toxin-BrnA
• Location: 4195701..4196276
• Replicon: chromosome
• Accession: NC_010681
• Organism: Burkholderia phytofirmans PsJN

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: B2T6W4
• Locus tag: BPHYT_RS18660
• Protein ID: WP_012434664.1
• Coordinates: 4195998..4196276 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: AbkA
• Uniprot ID: -
• Locus tag: BPHYT_RS18655
• Protein ID: WP_012434663.1
• Coordinates: 4195701..4195958 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPHYT_RS18635	4190888..4191640	+	753	WP_012434659.1	5-oxoprolinase subunit PxpA	-
BPHYT_RS18640	4191905..4192654	+	750	WP_012434660.1	DUF969 domain-containing protein	-
BPHYT_RS18645	4192651..4193607	+	957	WP_012434661.1	DUF979 domain-containing protein	-
BPHYT_RS18650	4193821..4195629	+	1809	WP_012434662.1	tetratricopeptide repeat protein	-
BPHYT_RS18655	4195701..4195958	-	258	WP_012434663.1	BrnA antitoxin family protein	Antitoxin
BPHYT_RS18660	4195998..4196276	-	279	WP_012434664.1	BrnT family toxin	Toxin
BPHYT_RS18665	4196477..4197640	-	1164	WP_012434665.1	TraB/GumN family protein	-
BPHYT_RS18670	4197633..4198652	-	1020	WP_012434666.1	ABC transporter ATP-binding protein	-
BPHYT_RS18675	4198649..4199653	-	1005	WP_012434667.1	ABC transporter ATP-binding protein	-
BPHYT_RS18680	4199654..4200571	-	918	WP_012434668.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (24-77)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 11097.67 Da
• Isoelectric Point: 6.9900

>T22869 WP_012434664.1 NC_010681:c4196276-4195998 [Paraburkholderia phytofirmans PsJN]
VLFEWDEIKNQINIRKHGIDFQDAIDVFNHPLLTALDRREDYGEDRWFAMGWIANIVGIVVYVERVEDVVRIISARKATR
HEVRHFKQRVWH

Nucleotide

• Length: 279 bp

>T22869 NC_010681:c4196276-4195998 [Paraburkholderia phytofirmans PsJN]
GTGCTGTTTGAATGGGACGAAATCAAAAATCAGATCAACATCCGCAAACACGGCATCGATTTTCAGGATGCTATCGACGT
GTTCAATCACCCGTTACTCACGGCGTTGGACCGGCGGGAAGACTACGGCGAAGACCGCTGGTTCGCGATGGGCTGGATCG
CAAACATTGTGGGCATCGTGGTGTATGTCGAGCGGGTCGAAGACGTGGTGCGGATCATTTCGGCACGCAAGGCAACGCGG
CACGAGGTCAGGCACTTCAAGCAGCGTGTCTGGCATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9890.25 Da
• Isoelectric Point: 9.6912

>AT22869 WP_012434663.1 NC_010681:c4195958-4195701 [Paraburkholderia phytofirmans PsJN]
MVKASGTDWKRLAKTDDAQIDTTDVPELGDDFFQRAELHVPPKQAVTMRLDADVLNWFKEQGQGYQTRINKLLRAYMLAQ
QRRRP

Nucleotide

• Length: 258 bp

>AT22869 NC_010681:c4195958-4195701 [Paraburkholderia phytofirmans PsJN]
ATGGTTAAGGCATCGGGAACAGACTGGAAGCGACTCGCCAAAACAGACGACGCGCAAATCGATACAACCGACGTGCCCGA
ACTGGGCGACGATTTTTTTCAACGCGCCGAGTTGCACGTTCCGCCGAAGCAGGCCGTGACAATGCGTCTGGATGCGGACG
TGCTGAACTGGTTCAAGGAGCAGGGGCAGGGTTATCAGACGCGCATCAACAAACTGTTGCGCGCCTATATGCTGGCGCAG
CAGCGGCGGCGTCCGTAA

Structures

Toxin

• AlphaFold DB: B2T6W4