Detailed information of TA system TA228681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242812..1243380
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LVJ67_RS05955
• Protein ID: WP_075928370.1
• Coordinates: 1243006..1243380 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ67_RS05950
• Protein ID: WP_003405863.1
• Coordinates: 1242812..1243009 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS05930 (LVJ67_05925)	1238853..1239491	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ67_RS05935 (LVJ67_05930)	1239581..1240588	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ67_RS05940 (LVJ67_05935)	1240605..1241588	-	984	WP_003898731.1	hypothetical protein	-
LVJ67_RS05945 (LVJ67_05940)	1241651..1242724	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ67_RS05950 (LVJ67_05945)	1242812..1243009	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ67_RS05955 (LVJ67_05950)	1243006..1243380	+	375	WP_075928370.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ67_RS05960 (LVJ67_05955)	1243583..1244281	+	699	WP_003898733.1	hypothetical protein	-
LVJ67_RS05965 (LVJ67_05960)	1244399..1244584	+	186	WP_003901093.1	hypothetical protein	-
LVJ67_RS05970 (LVJ67_05965)	1244511..1244786	-	276	WP_003405867.1	hypothetical protein	-
LVJ67_RS05975 (LVJ67_05970)	1245029..1245352	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ67_RS05980 (LVJ67_05975)	1245367..1246065	-	699	WP_031646953.1	hypothetical protein	-
LVJ67_RS05985 (LVJ67_05980)	1246099..1246308	+	210	WP_003911400.1	hypothetical protein	-
LVJ67_RS05990 (LVJ67_05985)	1246260..1247030	-	771	Protein_1180	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13430.53 Da
• Isoelectric Point: 5.1881

>T228681 WP_075928370.1 NZ_CP089772:1243006-1243380 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGGRLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T228681 NZ_CP124334:c2417153-2416980 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT228681 WP_003405863.1 NZ_CP089772:1242812-1243009 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT228681 NZ_CP124334:c2417477-2417181 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: G0TH07