Detailed information of TA system TA228677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1016719..1017338
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LVJ67_RS04820
• Protein ID: WP_003404726.1
• Coordinates: 1016904..1017338 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: LVJ67_RS04815
• Protein ID: WP_003898641.1
• Coordinates: 1016719..1016898 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS04800 (LVJ67_04795)	1012192..1013772	+	1581	WP_003909313.1	serine hydrolase	-
LVJ67_RS04805 (LVJ67_04800)	1013769..1016162	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
LVJ67_RS04810 (LVJ67_04805)	1016435..1016593	+	159	WP_003404720.1	hypothetical protein	-
LVJ67_RS04815 (LVJ67_04810)	1016719..1016898	+	180	WP_003898641.1	antitoxin	Antitoxin
LVJ67_RS04820 (LVJ67_04815)	1016904..1017338	+	435	WP_003404726.1	SRPBCC family protein	Toxin
LVJ67_RS04825 (LVJ67_04820)	1017436..1018209	+	774	WP_003404735.1	VOC family protein	-
LVJ67_RS04830 (LVJ67_04825)	1018274..1018723	+	450	WP_003404738.1	hypothetical protein	-
LVJ67_RS04835 (LVJ67_04830)	1018858..1019088	-	231	WP_003898642.1	hypothetical protein	-
LVJ67_RS04840 (LVJ67_04835)	1019255..1020763	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
LVJ67_RS04845 (LVJ67_04840)	1020765..1022003	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T228677 WP_003404726.1 NZ_CP089772:1016904-1017338 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T228677 NZ_CP124334:c1908907-1908593 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT228677 WP_003898641.1 NZ_CP089772:1016719-1016898 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT228677 NZ_CP124334:c1909143-1908910 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5