Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 756900..757692 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag LVJ67_RS03495 Protein ID WP_003403386.1
Coordinates 757255..757692 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag LVJ67_RS03490 Protein ID WP_003403381.1
Coordinates 756900..757145 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS03460 (LVJ67_03455) 751920..753425 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LVJ67_RS03465 (LVJ67_03460) 753506..754516 + 1011 WP_009937374.1 ABC transporter ATP-binding protein -
LVJ67_RS03470 (LVJ67_03465) 754904..755287 - 384 WP_003403365.1 ribonuclease VapC6 -
LVJ67_RS03475 (LVJ67_03470) 755382..755537 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ67_RS03480 (LVJ67_03475) 755613..756329 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ67_RS03485 (LVJ67_03480) 756605..756913 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
LVJ67_RS03490 (LVJ67_03485) 756900..757145 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ67_RS03495 (LVJ67_03490) 757255..757692 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ67_RS03500 (LVJ67_03495) 757689..757943 - 255 WP_003911263.1 antitoxin VapB7 -
LVJ67_RS03505 (LVJ67_03500) 758057..760420 + 2364 WP_003898547.1 arylsulfatase AtsD -
LVJ67_RS03510 (LVJ67_03505) 760483..760809 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ67_RS03515 (LVJ67_03510) 760721..761059 + 339 WP_003403405.1 PIN domain-containing protein -
LVJ67_RS03520 (LVJ67_03515) 761056..761229 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T228674 WP_003403386.1 NZ_CP089772:c757692-757255 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T228674 NZ_CP124334:c1467217-1466915 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT228674 WP_003403381.1 NZ_CP089772:c757145-756900 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT228674 NZ_CP124334:c1466879-1466538 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References