Detailed information of TA system TA228670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 717797..718446
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67241
• Locus tag: LVJ67_RS03285
• Protein ID: WP_003403236.1
• Coordinates: 718051..718446 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ34
• Locus tag: LVJ67_RS03280
• Protein ID: WP_003403235.1
• Coordinates: 717797..718051 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS03255 (LVJ67_03250)	712923..713006	+	84	Protein_645	galactose-1-phosphate uridylyltransferase	-
LVJ67_RS03260 (LVJ67_03255)	713025..714106	+	1082	Protein_646	galactose-1-phosphate uridylyltransferase	-
LVJ67_RS03265 (LVJ67_03260)	714103..715194	+	1092	WP_003906403.1	galactokinase	-
LVJ67_RS03270 (LVJ67_03265)	715589..716653	+	1065	WP_003898532.1	zinc ribbon domain-containing protein	-
LVJ67_RS03275 (LVJ67_03270)	716757..717704	+	948	WP_003403232.1	DUF732 domain-containing protein	-
LVJ67_RS03280 (LVJ67_03275)	717797..718051	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	Antitoxin
LVJ67_RS03285 (LVJ67_03280)	718051..718446	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	Toxin
LVJ67_RS03290 (LVJ67_03285)	718540..719280	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
LVJ67_RS03295 (LVJ67_03290)	719412..719672	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ67_RS03300 (LVJ67_03295)	719669..720076	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	-
LVJ67_RS03305 (LVJ67_03300)	720148..721299	-	1152	WP_003403248.1	FIST N-terminal domain-containing protein	-
LVJ67_RS03310 (LVJ67_03305)	721392..723119	-	1728	WP_003900979.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14250.04 Da
• Isoelectric Point: 4.7475

>T228670 WP_003403236.1 NZ_CP089772:718051-718446 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Nucleotide

• Length: 396 bp

>T228670 NZ_CP124333:95904-96320 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9139.52 Da
• Isoelectric Point: 9.2702

>AT228670 WP_003403235.1 NZ_CP089772:717797-718051 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Nucleotide

• Length: 255 bp

>AT228670 NZ_CP124333:95677-95907 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• PDB: 4XGR
• PDB: 4XGQ

Antitoxin

• AlphaFold DB: A0A7U4BSC2