Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 717797..718446 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag LVJ67_RS03285 Protein ID WP_003403236.1
Coordinates 718051..718446 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag LVJ67_RS03280 Protein ID WP_003403235.1
Coordinates 717797..718051 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS03255 (LVJ67_03250) 712923..713006 + 84 Protein_645 galactose-1-phosphate uridylyltransferase -
LVJ67_RS03260 (LVJ67_03255) 713025..714106 + 1082 Protein_646 galactose-1-phosphate uridylyltransferase -
LVJ67_RS03265 (LVJ67_03260) 714103..715194 + 1092 WP_003906403.1 galactokinase -
LVJ67_RS03270 (LVJ67_03265) 715589..716653 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ67_RS03275 (LVJ67_03270) 716757..717704 + 948 WP_003403232.1 DUF732 domain-containing protein -
LVJ67_RS03280 (LVJ67_03275) 717797..718051 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
LVJ67_RS03285 (LVJ67_03280) 718051..718446 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
LVJ67_RS03290 (LVJ67_03285) 718540..719280 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
LVJ67_RS03295 (LVJ67_03290) 719412..719672 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ67_RS03300 (LVJ67_03295) 719669..720076 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
LVJ67_RS03305 (LVJ67_03300) 720148..721299 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
LVJ67_RS03310 (LVJ67_03305) 721392..723119 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T228670 WP_003403236.1 NZ_CP089772:718051-718446 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T228670 NZ_CP124333:95904-96320 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT228670 WP_003403235.1 NZ_CP089772:717797-718051 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT228670 NZ_CP124333:95677-95907 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References