Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 712169..712794 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name vapC Uniprot ID G0TQA0
Locus tag LVJ67_RS03250 Protein ID WP_003403218.1
Coordinates 712393..712794 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ36
Locus tag LVJ67_RS03245 Protein ID WP_003403213.1
Coordinates 712169..712396 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS03220 (LVJ67_03215) 707348..707569 - 222 WP_003898525.1 DUF4926 domain-containing protein -
LVJ67_RS03225 (LVJ67_03220) 707711..708316 + 606 WP_003898526.1 hypothetical protein -
LVJ67_RS03230 (LVJ67_03225) 708335..710902 - 2568 WP_009937370.1 SEC-C metal-binding domain-containing protein -
LVJ67_RS03235 (LVJ67_03230) 710986..711735 + 750 WP_003898528.1 hypothetical protein -
LVJ67_RS03240 (LVJ67_03235) 711732..711974 + 243 WP_003403210.1 hypothetical protein -
LVJ67_RS03245 (LVJ67_03240) 712169..712396 + 228 WP_003403213.1 antitoxin VapB29 Antitoxin
LVJ67_RS03250 (LVJ67_03245) 712393..712794 + 402 WP_003403218.1 type II toxin-antitoxin system ribonuclease VapC29 Toxin
LVJ67_RS03255 (LVJ67_03250) 712923..713006 + 84 Protein_645 galactose-1-phosphate uridylyltransferase -
LVJ67_RS03260 (LVJ67_03255) 713025..714106 + 1082 Protein_646 galactose-1-phosphate uridylyltransferase -
LVJ67_RS03265 (LVJ67_03260) 714103..715194 + 1092 WP_003906403.1 galactokinase -
LVJ67_RS03270 (LVJ67_03265) 715589..716653 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ67_RS03275 (LVJ67_03270) 716757..717704 + 948 WP_003403232.1 DUF732 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-120)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 13937.76 Da        Isoelectric Point: 5.7441

>T228669 WP_003403218.1 NZ_CP089772:712393-712794 [Mycobacterium tuberculosis]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLIPTTT

Download         Length: 402 bp

>T228669 NZ_CP124333:c94897-94592 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8163.17 Da        Isoelectric Point: 5.6307

>AT228669 WP_003403213.1 NZ_CP089772:712169-712396 [Mycobacterium tuberculosis]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Download         Length: 228 bp

>AT228669 NZ_CP124333:c95117-94899 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQA0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSC9

References