Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/AbrB(antitoxin)
Location 698541..699187 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ88
Locus tag LVJ67_RS03145 Protein ID WP_003403137.1
Coordinates 698541..698954 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ89
Locus tag LVJ67_RS03150 Protein ID WP_003403139.1
Coordinates 698951..699187 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS03125 (LVJ67_03120) 694624..696174 + 1551 WP_003403119.1 MlaD family protein -
LVJ67_RS03130 (LVJ67_03125) 696226..696618 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 -
LVJ67_RS03135 (LVJ67_03130) 696615..696872 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 -
LVJ67_RS03140 (LVJ67_03135) 697055..698290 - 1236 WP_003403128.1 ATP-binding protein -
LVJ67_RS03145 (LVJ67_03140) 698541..698954 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ67_RS03150 (LVJ67_03145) 698951..699187 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 Antitoxin
LVJ67_RS03155 (LVJ67_03150) 699291..699797 - 507 WP_003900973.1 two component system sensor kinase HK1 -
LVJ67_RS03160 (LVJ67_03155) 699911..700441 - 531 WP_003917320.1 two component system sensor kinase HK2 -
LVJ67_RS03165 (LVJ67_03160) 700425..701120 - 696 WP_003918099.1 two-component system response regulator TcrA -
LVJ67_RS03170 (LVJ67_03165) 701243..701554 + 312 WP_003403164.1 hypothetical protein -
LVJ67_RS03175 (LVJ67_03170) 701626..702576 + 951 WP_003900184.1 DUF1259 domain-containing protein -
LVJ67_RS03180 (LVJ67_03175) 702817..703401 + 585 WP_003403171.1 IS607-like element IS1536 family transposase -
LVJ67_RS03185 (LVJ67_03180) 703403..704113 + 711 Protein_631 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-126)

Antitoxin

SpoVT-AbrB domain

(3-43)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14786.14 Da        Isoelectric Point: 8.1405

>T228667 WP_003403137.1 NZ_CP089772:c698954-698541 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Download         Length: 414 bp

>T228667 NZ_CP124332:c5212550-5212443 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8212.41 Da        Isoelectric Point: 5.0351

>AT228667 WP_003403139.1 NZ_CP089772:c699187-698951 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Download         Length: 237 bp

>AT228667 NZ_CP124332:5212598-5212664 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ88

Antitoxin

Source ID Structure
AlphaFold DB G0TQ89

References