Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 696226..696872 Replicon chromosome
Accession NZ_CP089772
Organism Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag LVJ67_RS03130 Protein ID WP_003403122.1
Coordinates 696226..696618 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag LVJ67_RS03135 Protein ID WP_003403125.1
Coordinates 696615..696872 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ67_RS03115 (LVJ67_03110) 691888..693414 + 1527 WP_003403112.1 virulence factor Mce family protein -
LVJ67_RS03120 (LVJ67_03115) 693477..694619 + 1143 WP_003911253.1 virulence factor Mce family protein -
LVJ67_RS03125 (LVJ67_03120) 694624..696174 + 1551 WP_003403119.1 MlaD family protein -
LVJ67_RS03130 (LVJ67_03125) 696226..696618 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
LVJ67_RS03135 (LVJ67_03130) 696615..696872 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
LVJ67_RS03140 (LVJ67_03135) 697055..698290 - 1236 WP_003403128.1 ATP-binding protein -
LVJ67_RS03145 (LVJ67_03140) 698541..698954 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
LVJ67_RS03150 (LVJ67_03145) 698951..699187 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
LVJ67_RS03155 (LVJ67_03150) 699291..699797 - 507 WP_003900973.1 two component system sensor kinase HK1 -
LVJ67_RS03160 (LVJ67_03155) 699911..700441 - 531 WP_003917320.1 two component system sensor kinase HK2 -
LVJ67_RS03165 (LVJ67_03160) 700425..701120 - 696 WP_003918099.1 two-component system response regulator TcrA -
LVJ67_RS03170 (LVJ67_03165) 701243..701554 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T228666 WP_003403122.1 NZ_CP089772:c696618-696226 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T228666 NZ_CP124332:4360945-4361247 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT228666 WP_003403125.1 NZ_CP089772:c696872-696615 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT228666 NZ_CP124332:4361283-4361624 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References