Detailed information of TA system TA228665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 679097..679716
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O53779
• Locus tag: LVJ67_RS03065
• Protein ID: WP_003403047.1
• Coordinates: 679309..679716 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O53778
• Locus tag: LVJ67_RS03060
• Protein ID: WP_003403039.1
• Coordinates: 679097..679312 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS03050 (LVJ67_03045)	677625..678383	+	759	WP_003898511.1	Mut7-C RNAse domain-containing protein	-
LVJ67_RS03055 (LVJ67_03050)	678512..679003	-	492	WP_003403017.1	mycobacterial cell wall protein Rv0580c	-
LVJ67_RS03060 (LVJ67_03055)	679097..679312	+	216	WP_003403039.1	type II toxin-antitoxin system antitoxin VapB26	Antitoxin
LVJ67_RS03065 (LVJ67_03060)	679309..679716	+	408	WP_003403047.1	type II toxin-antitoxin system toxin ribonuclease C26	Toxin
LVJ67_RS03070 (LVJ67_03065)	679776..680462	-	687	WP_003403052.1	LpqN/LpqT family lipoprotein	-
LVJ67_RS03075 (LVJ67_03070)	680616..683249	+	2634	WP_003403055.1	GH92 family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-39)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14471.43 Da
• Isoelectric Point: 4.4493

>T228665 WP_003403047.1 NZ_CP089772:679309-679716 [Mycobacterium tuberculosis]
VIIDTSALLAYFDAAEPDHAAVSECIDSSADALVVSPYVVAELDYLVATRVGVDAELAVLRELAGGAWELANCGAAEIEQ
AARIVTKYQDQRIGIADAANVVLADRYRTRTILTLDRRHFSALRPIGGGRFTVIP

Nucleotide

• Length: 408 bp

>T228665 NZ_CP124332:c4331988-4331770 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7572.66 Da
• Isoelectric Point: 10.5454

>AT228665 WP_003403039.1 NZ_CP089772:679097-679312 [Mycobacterium tuberculosis]
VDKTTVYLPDELKAAVKRAARQRGVSEAQVIRESIRAAVGGAKPPPRGGLYAGSEPIARRVDELLAGFGER

Nucleotide

• Length: 216 bp

>AT228665 NZ_CP124332:c4332388-4332014 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 5X3T

Antitoxin

• AlphaFold DB: A0A7U4BSA2