Detailed information of TA system TA228661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 367410..367981
• Replicon: chromosome
• Accession: NZ_CP089772
• Organism: Mycobacterium tuberculosis strain I0003165-3

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LVJ67_RS01595
• Protein ID: WP_003401560.1
• Coordinates: 367410..367712 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: LVJ67_RS01600
• Protein ID: WP_003401563.1
• Coordinates: 367760..367981 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ67_RS01575 (LVJ67_01575)	362879..363682	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
LVJ67_RS01580 (LVJ67_01580)	363692..365089	-	1398	WP_003401544.1	sulfatase	-
LVJ67_RS01585 (LVJ67_01585)	365268..367043	+	1776	WP_010886074.1	PE family protein	-
LVJ67_RS01590 (LVJ67_01590)	367186..367413	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
LVJ67_RS01595 (LVJ67_01595)	367410..367712	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LVJ67_RS01600 (LVJ67_01600)	367760..367981	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
LVJ67_RS01605 (LVJ67_01605)	367978..368403	+	426	WP_003401566.1	PIN domain nuclease	-
LVJ67_RS01610 (LVJ67_01610)	368539..369171	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
LVJ67_RS01615 (LVJ67_01615)	369168..370076	+	909	WP_003900117.1	protochlorophyllide reductase	-
LVJ67_RS01620 (LVJ67_01620)	370084..370674	-	591	WP_229298012.1	hypothetical protein	-
LVJ67_RS01625 (LVJ67_01625)	370667..370717	-	51	Protein_321	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T228661 WP_003401560.1 NZ_CP089772:367410-367712 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T228661 NZ_CP124332:3687193-3687570 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT228661 WP_003401563.1 NZ_CP089772:367760-367981 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT228661 NZ_CP124332:3686735-3687103 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48