228658

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4475908..4476498 Replicon chromosome
Accession NZ_CP089771
Organism Klebsiella pneumoniae strain HKE12

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag LVO50_RS21760 Protein ID WP_022615588.1
Coordinates 4475908..4476240 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag LVO50_RS21765 Protein ID WP_000288812.1
Coordinates 4476241..4476498 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO50_RS21735 (4471957) 4471957..4472202 + 246 WP_004141189.1 signal transduction protein PmrD -
LVO50_RS21740 (4472332) 4472332..4473786 + 1455 WP_004148975.1 AMP nucleosidase -
LVO50_RS21750 (4474152) 4474152..4475586 - 1435 Protein_4255 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LVO50_RS21760 (4475908) 4475908..4476240 - 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LVO50_RS21765 (4476241) 4476241..4476498 - 258 WP_000288812.1 antitoxin Antitoxin
LVO50_RS21770 (4476833) 4476833..4477039 - 207 WP_022615589.1 helix-turn-helix transcriptional regulator -
LVO50_RS21775 (4477036) 4477036..4477496 - 461 Protein_4259 hypothetical protein -
LVO50_RS21780 (4477779) 4477779..4478335 - 557 Protein_4260 hypothetical protein -
LVO50_RS21785 (4478414) 4478414..4480162 - 1749 WP_023302666.1 hypothetical protein -
LVO50_RS21790 (4480512) 4480512..4480589 - 78 Protein_4262 type II toxin-antitoxin system PemK/MazF family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T228658 WP_022615588.1 NZ_CP089771:c4476240-4475908 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T228658 NZ_CP124332:3370985-3371158 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT228658 WP_000288812.1 NZ_CP089771:c4476498-4476241 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT228658 NZ_CP124332:3370661-3370957 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References