Detailed information of TA system TA228649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3602318..3603906
• Replicon: chromosome
• Accession: NZ_CP089771
• Organism: Klebsiella pneumoniae strain HKE12

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LVO50_RS17410
• Protein ID: WP_004213069.1
• Coordinates: 3602584..3603906 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LVO50_RS17405
• Protein ID: WP_004176198.1
• Coordinates: 3602318..3602584 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVO50_RS17380 (3597348)	3597348..3598271	+	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-
LVO50_RS17385 (3598252)	3598252..3599406	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LVO50_RS17390 (3599752)	3599752..3601131	+	1380	WP_004215088.1	nucleobase:cation symporter-2 family protein	-
LVO50_RS17395 (3601109)	3601109..3601609	+	501	WP_004215086.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LVO50_RS17400 (3601606)	3601606..3601932	+	327	WP_004215084.1	hydroxyisourate hydrolase	-
LVO50_RS17405 (3602318)	3602318..3602584	+	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LVO50_RS17410 (3602584)	3602584..3603906	+	1323	WP_004213069.1	type II toxin-antitoxin system HipA family toxin	Toxin
LVO50_RS17415 (3603923)	3603923..3605215	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LVO50_RS17420 (3605357)	3605357..3606361	-	1005	WP_004213068.1	fimbrial protein	-
LVO50_RS17425 (3606349)	3606349..3608850	-	2502	WP_004213067.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49128.44 Da
• Isoelectric Point: 7.5357

>T228649 WP_004213069.1 NZ_CP089771:3602584-3603906 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQATGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T228649 NZ_CP124332:c919182-918931 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT228649 WP_004176198.1 NZ_CP089771:3602318-3602584 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT228649 NZ_CP124332:c918934-918680 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA