Detailed information of TA system TA228641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 87089..87582
• Replicon: plasmid unnamed2
• Accession: NZ_CP089770
• Organism: Klebsiella pneumoniae strain HKE9

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: LVO52_RS26965
• Protein ID: WP_001230709.1
• Coordinates: 87328..87582 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: LVO52_RS26960
• Protein ID: WP_000151456.1
• Coordinates: 87089..87331 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVO52_RS26930 (LVO52_26900)	82412..82705	-	294	WP_000954637.1	hypothetical protein	-
LVO52_RS26935 (LVO52_26905)	82720..83094	-	375	WP_015387341.1	hypothetical protein	-
LVO52_RS26940 (LVO52_26910)	83146..83433	-	288	WP_000673430.1	hypothetical protein	-
LVO52_RS26945 (LVO52_26915)	84183..85445	+	1263	WP_000608644.1	IS1380-like element ISEcp1 family transposase	-
LVO52_RS26950 (LVO52_26920)	85698..86573	+	876	WP_015387340.1	extended-spectrum class A beta-lactamase CTX-M-55	-
LVO52_RS26955 (LVO52_26925)	86620..86964	-	345	Protein_96	WbuC family cupin fold metalloprotein	-
LVO52_RS26960 (LVO52_26930)	87089..87331	-	243	WP_000151456.1	hypothetical protein	Antitoxin
LVO52_RS26965 (LVO52_26935)	87328..87582	-	255	WP_001230709.1	hypothetical protein	Toxin
LVO52_RS26970 (LVO52_26940)	87579..88121	-	543	WP_001083904.1	hypothetical protein	-
LVO52_RS26975 (LVO52_26945)	88225..88740	-	516	WP_001025393.1	J domain-containing protein	-
LVO52_RS26980 (LVO52_26950)	89144..89473	-	330	WP_000855119.1	hypothetical protein	-
LVO52_RS26985 (LVO52_26955)	90060..90725	+	666	WP_015387337.1	division plane positioning ATPase MipZ	-
LVO52_RS26990 (LVO52_26960)	90799..91083	+	285	WP_001162371.1	hypothetical protein	-
LVO52_RS26995 (LVO52_26965)	91101..91697	-	597	WP_000125165.1	ProQ/FINO family protein	-
LVO52_RS27000 (LVO52_26970)	91846..91968	-	123	WP_223202818.1	Hok/Gef family protein	-
LVO52_RS27005 (LVO52_26975)	91913..92065	-	153	Protein_106	DUF5431 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-33 / blaCTX-M-55	-	1..99554	99554
	-	flank	IS/Tn	blaCTX-M-55	-	84183..86573	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T228641 WP_001230709.1 NZ_CP089770:c87582-87328 [Klebsiella pneumoniae]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T228641 NZ_CP124329:c138489-138184 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT228641 WP_000151456.1 NZ_CP089770:c87331-87089 [Klebsiella pneumoniae]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT228641 NZ_CP124329:c138709-138491 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5