Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 116163..117073 Replicon plasmid unnamed1
Accession NZ_CP089769
Organism Klebsiella pneumoniae strain HKE9

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag LVO52_RS26235 Protein ID WP_004144067.1
Coordinates 116603..117073 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID W9B1L8
Locus tag LVO52_RS26230 Protein ID WP_016528982.1
Coordinates 116163..116606 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO52_RS26200 (LVO52_26170) 111177..111698 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
LVO52_RS26205 (LVO52_26175) 111695..112648 + 954 Protein_118 fec operon regulator FecR -
LVO52_RS26210 (LVO52_26180) 112735..114861 + 2127 WP_032446634.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
LVO52_RS26215 (LVO52_26185) 114943..115068 + 126 Protein_120 Fe3+-citrate ABC transporter substrate-binding protein -
LVO52_RS26220 (LVO52_26190) 115243..115464 + 222 WP_016528984.1 DUF2188 domain-containing protein -
LVO52_RS26225 (LVO52_26195) 115573..116062 + 490 Protein_122 DUF4113 domain-containing protein -
LVO52_RS26230 (LVO52_26200) 116163..116606 + 444 WP_016528982.1 DUF2384 domain-containing protein Antitoxin
LVO52_RS26235 (LVO52_26205) 116603..117073 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
LVO52_RS26240 (LVO52_26210) 117184..117444 - 261 WP_016528980.1 hypothetical protein -
LVO52_RS26245 (LVO52_26215) 117844..119072 + 1229 WP_085956323.1 IS3 family transposase -
LVO52_RS26250 (LVO52_26220) 119043..119822 - 780 WP_225932725.1 Hok/Gef family protein -
LVO52_RS26255 (LVO52_26225) 120308..120475 + 168 WP_162837823.1 hypothetical protein -
LVO52_RS26260 (LVO52_26230) 121116..121274 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
LVO52_RS26265 (LVO52_26235) 121759..121965 + 207 Protein_130 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iucA / iucB / iucC / iucD / iutA / iroB / iroC / iroD / iroN / rmpA 1..161792 161792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(94-144)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T228640 WP_004144067.1 NZ_CP089769:116603-117073 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T228640 NZ_CP124329:c76673-76524 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16494.83 Da        Isoelectric Point: 10.2498

>AT228640 WP_016528982.1 NZ_CP089769:116163-116606 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEIIKLITRLEHGVYS

Download         Length: 444 bp

>AT228640 NZ_CP124329:76717-76778 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB W9B1L8

References