Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 204009..204784 Replicon plasmid pBS512_211
Accession NC_010660
Organism Shigella boydii CDC 3083-94

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag SBBS512_RS01630 Protein ID WP_000405248.1
Coordinates 204302..204784 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag SBBS512_RS01625 Protein ID WP_010921625.1
Coordinates 204009..204311 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SBBS512_RS01595 199060..200514 + 1455 WP_000701110.1 type 3 secretion system effector OspC2 -
SBBS512_RS33490 200663..200791 - 129 Protein_238 IS5/IS1182 family transposase -
SBBS512_RS01600 200963..202057 - 1095 Protein_239 IS91 family transposase -
SBBS512_RS01610 202109..203322 + 1214 WP_136952279.1 IS3 family transposase -
SBBS512_RS01620 203465..203737 - 273 WP_000019158.1 hypothetical protein -
SBBS512_RS01625 204009..204311 + 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
SBBS512_RS01630 204302..204784 + 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
SBBS512_RS30940 204953..206029 + 1077 Protein_244 IS3 family transposase -
SBBS512_RS01645 206053..206745 - 693 WP_000625263.1 type III secretion system effector cysteine methyltransferase OspZ -
SBBS512_RS27965 206801..206953 + 153 Protein_246 IS3 family transposase -
SBBS512_RS01660 208334..209281 + 948 WP_005005342.1 omptin family outer membrane protease IcsP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2 / ospD3/senA / ospC1 / senB / ipaH7.8 / ipaH4.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA 1..210919 210919
- inside IScluster/Tn - ospC2 / nleE / icsP/sopA 190927..210769 19842


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T22864 WP_000405248.1 NC_010660:204302-204784 [Shigella boydii CDC 3083-94]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T22864 NC_010660:204302-204784 [Shigella boydii CDC 3083-94]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT22864 WP_010921625.1 NC_010660:204009-204311 [Shigella boydii CDC 3083-94]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT22864 NC_010660:204009-204311 [Shigella boydii CDC 3083-94]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCTTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References