Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 55610..56361 Replicon plasmid unnamed1
Accession NZ_CP089769
Organism Klebsiella pneumoniae strain HKE9

Toxin (Protein)


Gene name ataT Uniprot ID W9BA31
Locus tag LVO52_RS25905 Protein ID WP_032104592.1
Coordinates 55879..56361 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID W9B1S8
Locus tag LVO52_RS25900 Protein ID WP_016529519.1
Coordinates 55610..55888 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO52_RS25880 (LVO52_25850) 51829..52911 - 1083 WP_016528990.1 DNA-binding transcriptional repressor LacI -
LVO52_RS25885 (LVO52_25855) 52947..53029 - 83 Protein_54 hypothetical protein -
LVO52_RS25890 (LVO52_25860) 53372..54382 + 1011 WP_004152284.1 zinc-binding alcohol dehydrogenase family protein -
LVO52_RS25895 (LVO52_25865) 54766..55302 - 537 WP_032445791.1 hypothetical protein -
LVO52_RS25900 (LVO52_25870) 55610..55888 + 279 WP_016529519.1 DUF1778 domain-containing protein Antitoxin
LVO52_RS25905 (LVO52_25875) 55879..56361 + 483 WP_032104592.1 GNAT family N-acetyltransferase Toxin
LVO52_RS25910 (LVO52_25880) 56399..56803 + 405 WP_016529521.1 DUF2251 domain-containing protein -
LVO52_RS25915 (LVO52_25885) 57140..57973 + 834 WP_012540133.1 GIY-YIG nuclease family protein -
LVO52_RS25920 (LVO52_25890) 58073..58234 + 162 WP_223367960.1 bacteriocin immunity protein -
LVO52_RS25925 (LVO52_25895) 58329..58565 - 237 WP_016529523.1 hypothetical protein -
LVO52_RS25935 (LVO52_25905) 60326..60778 - 453 WP_020948374.1 transcriptional repressor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iucA / iucB / iucC / iucD / iutA / iroB / iroC / iroD / iroN / rmpA 1..161792 161792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-144)

Antitoxin

Vibrio phage ICP1, Orf50

(6-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17781.73 Da        Isoelectric Point: 9.4946

>T228639 WP_032104592.1 NZ_CP089769:55879-56361 [Klebsiella pneumoniae]
MGMRPPEPLTPEHNIAEFCCQDQVLSEWLKKKALKNHRTGISRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTEHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Download         Length: 483 bp

>T228639 NZ_CP124328:c5212293-5212186 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10708.23 Da        Isoelectric Point: 4.5156

>AT228639 WP_016529519.1 NZ_CP089769:55610-55888 [Klebsiella pneumoniae]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDNQFNAFVEELDAPMTPERQAR
IDNLMSRKSPWE

Download         Length: 279 bp

>AT228639 NZ_CP124328:5212341-5212407 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BA31

Antitoxin

Source ID Structure
AlphaFold DB W9B1S8

References