Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4815841..4816466 Replicon chromosome
Accession NZ_CP089768
Organism Klebsiella pneumoniae strain HKE9

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LVO52_RS23275 Protein ID WP_002882817.1
Coordinates 4815841..4816224 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LVO52_RS23280 Protein ID WP_004150355.1
Coordinates 4816224..4816466 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO52_RS23260 (4813207) 4813207..4814109 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LVO52_RS23265 (4814106) 4814106..4814741 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LVO52_RS23270 (4814738) 4814738..4815667 + 930 WP_058229092.1 formate dehydrogenase accessory protein FdhE -
LVO52_RS23275 (4815841) 4815841..4816224 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LVO52_RS23280 (4816224) 4816224..4816466 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LVO52_RS23285 (4816671) 4816671..4817588 + 918 WP_004181612.1 alpha/beta hydrolase -
LVO52_RS23290 (4817602) 4817602..4818543 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LVO52_RS23295 (4818588) 4818588..4819025 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LVO52_RS23300 (4819022) 4819022..4819882 - 861 WP_002882807.1 virulence factor BrkB family protein -
LVO52_RS23305 (4819876) 4819876..4820475 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T228637 WP_002882817.1 NZ_CP089768:c4816224-4815841 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T228637 NZ_CP124328:c4036086-4035709 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT228637 WP_004150355.1 NZ_CP089768:c4816466-4816224 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT228637 NZ_CP124328:c4036543-4036175 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References