Detailed information of TA system TA228631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2287335..2288923
• Replicon: chromosome
• Accession: NZ_CP089768
• Organism: Klebsiella pneumoniae strain HKE9

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LVO52_RS11240
• Protein ID: WP_040148030.1
• Coordinates: 2287335..2288657 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LVO52_RS11245
• Protein ID: WP_002904790.1
• Coordinates: 2288657..2288923 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVO52_RS11225 (2282391)	2282391..2284892	+	2502	WP_064188738.1	fimbria/pilus outer membrane usher protein	-
LVO52_RS11230 (2284880)	2284880..2285884	+	1005	WP_009308876.1	fimbrial protein	-
LVO52_RS11235 (2286026)	2286026..2287318	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LVO52_RS11240 (2287335)	2287335..2288657	-	1323	WP_040148030.1	type II toxin-antitoxin system HipA family toxin	Toxin
LVO52_RS11245 (2288657)	2288657..2288923	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LVO52_RS11250 (2289309)	2289309..2289635	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
LVO52_RS11255 (2289632)	2289632..2290132	-	501	WP_046654191.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LVO52_RS11260 (2290110)	2290110..2291489	-	1380	WP_004184021.1	nucleobase:cation symporter-2 family protein	-
LVO52_RS11265 (2291835)	2291835..2292988	+	1154	Protein_2202	FAD-dependent urate hydroxylase HpxO	-
LVO52_RS11270 (2292969)	2292969..2293892	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5366

>T228631 WP_040148030.1 NZ_CP089768:c2288657-2287335 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T228631 NZ_CP124328:c3017272-3016961 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT228631 WP_002904790.1 NZ_CP089768:c2288923-2288657 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT228631 NZ_CP124328:c3016960-3016670 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8