Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 182254..182879 Replicon plasmid pBS512_211
Accession NC_010660
Organism Shigella boydii CDC 3083-94

Toxin (Protein)


Gene name vapC Uniprot ID A0A2S8DLI0
Locus tag SBBS512_RS01490 Protein ID WP_000911319.1
Coordinates 182254..182652 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag SBBS512_RS01495 Protein ID WP_000450530.1
Coordinates 182652..182879 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SBBS512_RS01460 177308..178252 + 945 WP_012421740.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
SBBS512_RS01465 178407..179078 - 672 Protein_214 IS630 family transposase -
SBBS512_RS01470 179094..179791 - 698 Protein_215 IS1 family transposase -
SBBS512_RS01485 180039..182245 + 2207 Protein_216 type IV conjugative transfer system coupling protein TraD -
SBBS512_RS01490 182254..182652 - 399 WP_000911319.1 tRNA(fMet)-specific endonuclease VapC Toxin
SBBS512_RS01495 182652..182879 - 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
SBBS512_RS33695 183083..183268 + 186 WP_171537368.1 relaxase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2 / ospD3/senA / ospC1 / senB / ipaH7.8 / ipaH4.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA 1..210919 210919
- inside IScluster/Tn - ipaH9.8 / ospG 149861..179597 29736


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14887.17 Da        Isoelectric Point: 8.5264

>T22863 WP_000911319.1 NC_010660:c182652-182254 [Shigella boydii CDC 3083-94]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
VQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T22863 NC_010660:c182652-182254 [Shigella boydii CDC 3083-94]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GTCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT22863 WP_000450530.1 NC_010660:c182879-182652 [Shigella boydii CDC 3083-94]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT22863 NC_010660:c182879-182652 [Shigella boydii CDC 3083-94]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S8DLI0

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References