228622

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1373782..1374372 Replicon chromosome
Accession NZ_CP089768
Organism Klebsiella pneumoniae strain HKE9

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag LVO52_RS06680 Protein ID WP_022615588.1
Coordinates 1374040..1374372 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag LVO52_RS06675 Protein ID WP_000288812.1
Coordinates 1373782..1374039 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO52_RS06650 (1369690) 1369690..1369767 + 78 Protein_1298 type II toxin-antitoxin system PemK/MazF family toxin -
LVO52_RS06655 (1370118) 1370118..1371866 + 1749 WP_277695948.1 hypothetical protein -
LVO52_RS06660 (1371945) 1371945..1372501 + 557 Protein_1300 hypothetical protein -
LVO52_RS06665 (1372784) 1372784..1373244 + 461 Protein_1301 hypothetical protein -
LVO52_RS06670 (1373241) 1373241..1373447 + 207 WP_020805021.1 helix-turn-helix transcriptional regulator -
LVO52_RS06675 (1373782) 1373782..1374039 + 258 WP_000288812.1 antitoxin Antitoxin
LVO52_RS06680 (1374040) 1374040..1374372 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LVO52_RS06690 (1374694) 1374694..1376130 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LVO52_RS06700 (1376496) 1376496..1377950 - 1455 WP_004148975.1 AMP nucleosidase -
LVO52_RS06705 (1378080) 1378080..1378325 - 246 WP_023284006.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T228622 WP_022615588.1 NZ_CP089768:1374040-1374372 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T228622 NZ_CP124328:c1711096-1710794 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT228622 WP_000288812.1 NZ_CP089768:1373782-1374039 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT228622 NZ_CP124328:c1710758-1710417 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References