Detailed information of TA system TA228621

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 412838..413538
• Replicon: chromosome
• Accession: NZ_CP089768
• Organism: Klebsiella pneumoniae strain HKE9

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7H4NX47
• Locus tag: LVO52_RS02200
• Protein ID: WP_001546951.1
• Coordinates: 413215..413538 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LVO52_RS02195
• Protein ID: WP_049116113.1
• Coordinates: 412838..413194 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVO52_RS02165 (408790)	408790..409683	+	894	WP_048215606.1	50S ribosome-binding GTPase	-
LVO52_RS02170 (409968)	409968..410645	+	678	WP_048215607.1	hypothetical protein	-
LVO52_RS02175 (410744)	410744..411565	+	822	WP_048215608.1	DUF932 domain-containing protein	-
LVO52_RS02180 (411596)	411596..412039	+	444	WP_048215609.1	antirestriction protein	-
LVO52_RS02185 (412052)	412052..412594	+	543	WP_048215610.1	DNA repair protein RadC	-
LVO52_RS02190 (412591)	412591..412812	+	222	WP_047644100.1	DUF987 family protein	-
LVO52_RS02195 (412838)	412838..413194	+	357	WP_049116113.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LVO52_RS02200 (413215)	413215..413538	+	324	WP_001546951.1	TA system toxin CbtA family protein	Toxin
LVO52_RS02205 (413914)	413914..415302	-	1389	WP_087787964.1	MFS transporter	-
LVO52_RS02210 (415432)	415432..416361	+	930	WP_004185541.1	LysR family transcriptional regulator	-
LVO52_RS02215 (416485)	416485..416856	-	372	Protein_430	IS3 family transposase	-
LVO52_RS02220 (416965)	416965..418182	-	1218	WP_277695865.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	392249..429065	36816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12496.55 Da
• Isoelectric Point: 7.1331

>T228621 WP_001546951.1 NZ_CP089768:413215-413538 [Klebsiella pneumoniae]
MKFLPTTNMRAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLMNRN

Nucleotide

• Length: 324 bp

>T228621 NZ_CP124328:c919164-918913 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13083.78 Da
• Isoelectric Point: 6.2018

>AT228621 WP_049116113.1 NZ_CP089768:412838-413194 [Klebsiella pneumoniae]
MNNHSESGAIPENPPCQQWGLKSTITPCFGARLVQEGDRLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELILTSGE
LSPRHQHCVTLYHNGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 357 bp

>AT228621 NZ_CP124328:c918916-918662 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A7H4NX47