Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 169187..169712 | Replicon | plasmid pBS512_211 |
| Accession | NC_010660 | ||
| Organism | Shigella boydii CDC 3083-94 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | Q326Z8 |
| Locus tag | SBBS512_RS01410 | Protein ID | WP_001159860.1 |
| Coordinates | 169407..169712 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | Q7BEK0 |
| Locus tag | SBBS512_RS01405 | Protein ID | WP_000813626.1 |
| Coordinates | 169187..169405 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SBBS512_RS01370 | 164928..165677 | - | 750 | WP_000828661.1 | phosphatase PAP2 family protein | - |
| SBBS512_RS27900 | 166040..166273 | + | 234 | Protein_198 | DDE-type integrase/transposase/recombinase | - |
| SBBS512_RS01380 | 166286..166978 | + | 693 | Protein_199 | group II intron reverse transcriptase/maturase | - |
| SBBS512_RS01390 | 167033..168246 | + | 1214 | WP_167549322.1 | IS3 family transposase | - |
| SBBS512_RS01395 | 168322..168594 | - | 273 | WP_012421738.1 | hypothetical protein | - |
| SBBS512_RS01405 | 169187..169405 | + | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| SBBS512_RS01410 | 169407..169712 | + | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| SBBS512_RS32765 | 169748..169843 | + | 96 | Protein_204 | transposase domain-containing protein | - |
| SBBS512_RS01415 | 170105..171742 | + | 1638 | WP_012421769.1 | T3SS effector E3 ubiquitin-protein ligase IpaH9.8 | - |
| SBBS512_RS01420 | 171950..172219 | + | 270 | WP_000079956.1 | hypothetical protein | - |
| SBBS512_RS01425 | 172471..172956 | + | 486 | WP_000705603.1 | hypothetical protein | - |
| SBBS512_RS01430 | 172971..173668 | - | 698 | Protein_208 | IS1 family transposase | - |
| SBBS512_RS01440 | 173875..173991 | + | 117 | WP_004996477.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2 / ospD3/senA / ospC1 / senB / ipaH7.8 / ipaH4.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA | 1..210919 | 210919 | |
| - | inside | IScluster/Tn | - | ipaH9.8 / ospG | 149861..179597 | 29736 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11736.59 Da Isoelectric Point: 6.4674
>T22862 WP_001159860.1 NC_010660:169407-169712 [Shigella boydii CDC 3083-94]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T22862 NC_010660:169407-169712 [Shigella boydii CDC 3083-94]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTTTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTTTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8333.36 Da Isoelectric Point: 6.2838
>AT22862 WP_000813626.1 NC_010660:169187-169405 [Shigella boydii CDC 3083-94]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
Download Length: 219 bp
>AT22862 NC_010660:169187-169405 [Shigella boydii CDC 3083-94]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TTN3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q7BEK0 |