Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 49225..49868 Replicon plasmid unnamed2
Accession NZ_CP089767
Organism Klebsiella pneumoniae strain HKC2

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag LVO53_RS27060 Protein ID WP_000754566.1
Coordinates 49225..49641 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LVO53_RS27065 Protein ID WP_001261276.1
Coordinates 49638..49868 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO53_RS27015 (LVO53_26970) 44247..44504 + 258 WP_004152553.1 cloacin immunity family protein -
LVO53_RS27020 (LVO53_26975) 44681..45541 - 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
LVO53_RS27025 (LVO53_26980) 45724..46017 - 294 Protein_55 helix-turn-helix domain-containing protein -
LVO53_RS27030 (LVO53_26985) 46016..46123 - 108 Protein_56 class D beta-lactamase -
LVO53_RS27035 (LVO53_26990) 46123..46991 - 869 Protein_57 AadA family aminoglycoside 3''-O-nucleotidyltransferase -
LVO53_RS27040 (LVO53_26995) 46997..47287 - 291 WP_000470556.1 DUF1010 domain-containing protein -
LVO53_RS27045 (LVO53_27000) 47443..47901 - 459 WP_013263788.1 aminoglycoside N-acetyltransferase AAC(6')-Il -
LVO53_RS27050 (LVO53_27005) 48048..48362 + 315 Protein_60 class 1 integron integrase IntI1 -
LVO53_RS27055 (LVO53_27010) 48440..49144 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LVO53_RS27060 (LVO53_27015) 49225..49641 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
LVO53_RS27065 (LVO53_27020) 49638..49868 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVO53_RS27070 (LVO53_27025) 50457..50750 + 294 WP_020956883.1 hypothetical protein -
LVO53_RS27075 (LVO53_27030) 50814..51500 + 687 WP_020956882.1 hypothetical protein -
LVO53_RS27080 (LVO53_27035) 51512..52288 + 777 WP_020956881.1 tyrosine-type recombinase/integrase -
LVO53_RS27085 (LVO53_27040) 52346..52603 - 258 WP_000764642.1 hypothetical protein -
LVO53_RS27090 (LVO53_27045) 52732..52836 - 105 WP_032409716.1 hypothetical protein -
LVO53_RS27095 (LVO53_27050) 53366..54232 + 867 WP_004118283.1 replication initiation protein -
LVO53_RS27100 (LVO53_27055) 54409..54678 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1A / ant(3'')-Ia / aac(6')-Il - 1..68291 68291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T228619 WP_000754566.1 NZ_CP089767:c49641-49225 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T228619 NZ_CP124328:866660-867034 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT228619 WP_001261276.1 NZ_CP089767:c49868-49638 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT228619 NZ_CP124328:866203-866571 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References