Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14600..15243 Replicon plasmid unnamed2
Accession NZ_CP089767
Organism Klebsiella pneumoniae strain HKC2

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag LVO53_RS26855 Protein ID WP_000754566.1
Coordinates 14600..15016 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LVO53_RS26860 Protein ID WP_001261276.1
Coordinates 15013..15243 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO53_RS26810 (LVO53_26765) 9622..9879 + 258 WP_004152553.1 cloacin immunity family protein -
LVO53_RS26815 (LVO53_26770) 10056..10916 - 861 WP_277709812.1 TEM family class A beta-lactamase -
LVO53_RS26820 (LVO53_26775) 11099..11392 - 294 Protein_14 helix-turn-helix domain-containing protein -
LVO53_RS26825 (LVO53_26780) 11391..11498 - 108 Protein_15 class D beta-lactamase -
LVO53_RS26830 (LVO53_26785) 11498..12366 - 869 Protein_16 AadA family aminoglycoside 3''-O-nucleotidyltransferase -
LVO53_RS26835 (LVO53_26790) 12372..12662 - 291 WP_000470556.1 DUF1010 domain-containing protein -
LVO53_RS26840 (LVO53_26795) 12818..13276 - 459 WP_013263788.1 aminoglycoside N-acetyltransferase AAC(6')-Il -
LVO53_RS26845 (LVO53_26800) 13423..13737 + 315 Protein_19 class 1 integron integrase IntI1 -
LVO53_RS26850 (LVO53_26805) 13815..14519 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LVO53_RS26855 (LVO53_26810) 14600..15016 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
LVO53_RS26860 (LVO53_26815) 15013..15243 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVO53_RS26865 (LVO53_26820) 15832..16125 + 294 WP_020956883.1 hypothetical protein -
LVO53_RS26870 (LVO53_26825) 16189..16875 + 687 WP_020956882.1 hypothetical protein -
LVO53_RS26875 (LVO53_26830) 16887..17663 + 777 WP_020956881.1 tyrosine-type recombinase/integrase -
LVO53_RS26880 (LVO53_26835) 17721..17978 - 258 WP_000764642.1 hypothetical protein -
LVO53_RS26885 (LVO53_26840) 18107..18211 - 105 WP_032409716.1 hypothetical protein -
LVO53_RS26890 (LVO53_26845) 18741..19607 + 867 WP_004118283.1 replication initiation protein -
LVO53_RS26895 (LVO53_26850) 19784..20053 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1A / ant(3'')-Ia / aac(6')-Il - 1..68291 68291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T228618 WP_000754566.1 NZ_CP089767:c15016-14600 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T228618 NZ_CP124328:c515024-514922 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT228618 WP_001261276.1 NZ_CP089767:c15243-15013 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT228618 NZ_CP124328:514884-515152 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References