Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 19231..19967 Replicon plasmid unnamed1
Accession NZ_CP089766
Organism Klebsiella pneumoniae strain HKC2

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LVO53_RS25935 Protein ID WP_003026803.1
Coordinates 19485..19967 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LVO53_RS25930 Protein ID WP_003026799.1
Coordinates 19231..19497 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO53_RS25885 (LVO53_25845) 15293..15655 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
LVO53_RS25890 (LVO53_25850) 15705..16055 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LVO53_RS25895 (LVO53_25855) 16413..16682 + 270 WP_004152102.1 hypothetical protein -
LVO53_RS25900 (LVO53_25860) 16670..17245 + 576 WP_004152103.1 hypothetical protein -
LVO53_RS25905 (LVO53_25865) 17276..17770 + 495 WP_004152104.1 DNA-binding protein -
LVO53_RS25910 (LVO53_25870) 17814..18182 + 369 WP_004152105.1 hypothetical protein -
LVO53_RS25915 (LVO53_25875) 18216..18419 + 204 WP_004152106.1 HHA domain-containing protein -
LVO53_RS25920 (LVO53_25880) 18468..18725 + 258 WP_004152107.1 hypothetical protein -
LVO53_RS25925 (LVO53_25885) 18801..19055 + 255 WP_004152108.1 hypothetical protein -
LVO53_RS25930 (LVO53_25890) 19231..19497 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LVO53_RS25935 (LVO53_25895) 19485..19967 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LVO53_RS25940 (LVO53_25900) 20179..21525 + 1347 WP_020314316.1 ISNCY family transposase -
LVO53_RS25945 21686..21817 + 132 WP_004218042.1 hypothetical protein -
LVO53_RS25950 (LVO53_25905) 22026..23191 - 1166 Protein_30 IS3 family transposase -
LVO53_RS25955 (LVO53_25910) 23368..24330 - 963 WP_134892070.1 zinc metalloprotease HtpX -
LVO53_RS25960 (LVO53_25915) 24317..24805 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / sul1 - 1..182496 182496


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T228617 WP_003026803.1 NZ_CP089766:19485-19967 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T228617 NZ_CP124328:c234347-233025 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT228617 WP_003026799.1 NZ_CP089766:19231-19497 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT228617 NZ_CP124328:c234613-234347 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References