Detailed information of TA system TA228616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4471937..4473525
• Replicon: chromosome
• Accession: NZ_CP089765
• Organism: Klebsiella pneumoniae strain HKC2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LVO53_RS21870
• Protein ID: WP_004179815.1
• Coordinates: 4471937..4473259 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LVO53_RS21875
• Protein ID: WP_004176198.1
• Coordinates: 4473259..4473525 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVO53_RS21855 (4466993)	4466993..4469494	+	2502	WP_004179823.1	fimbria/pilus outer membrane usher protein	-
LVO53_RS21860 (4469482)	4469482..4470486	+	1005	WP_032443455.1	fimbrial protein	-
LVO53_RS21865 (4470628)	4470628..4471920	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LVO53_RS21870 (4471937)	4471937..4473259	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
LVO53_RS21875 (4473259)	4473259..4473525	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LVO53_RS21880 (4473791)	4473791..4474117	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
LVO53_RS21885 (4474114)	4474114..4474614	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LVO53_RS21890 (4474592)	4474592..4475971	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
LVO53_RS21895 (4476317)	4476317..4477471	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LVO53_RS21900 (4477452)	4477452..4478375	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	4267927..4508361	240434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T228616 WP_004179815.1 NZ_CP089765:c4473259-4471937 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T228616 NZ_CP124327:c142262-141957 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT228616 WP_004176198.1 NZ_CP089765:c4473525-4473259 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT228616 NZ_CP124327:c142482-142264 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA