Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 40092..40849 Replicon plasmid pBS512_211
Accession NC_010660
Organism Shigella boydii CDC 3083-94

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag SBBS512_RS00610 Protein ID WP_000501969.1
Coordinates 40092..40577 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag SBBS512_RS00615 Protein ID WP_011114751.1
Coordinates 40565..40849 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SBBS512_RS00570 35587..35766 - 180 Protein_47 transposase -
SBBS512_RS30775 35803..37012 - 1210 Protein_48 IS3 family transposase -
SBBS512_RS00590 37314..37610 + 297 WP_039059935.1 helix-turn-helix domain-containing protein -
SBBS512_RS00595 37662..38081 + 420 WP_000435428.1 IS66 family insertion sequence hypothetical protein -
SBBS512_RS00600 38078..38428 + 351 Protein_51 IS66 family insertion sequence element accessory protein TnpB -
SBBS512_RS27620 38459..40051 + 1593 WP_001072392.1 IS66 family transposase -
SBBS512_RS00610 40092..40577 - 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
SBBS512_RS00615 40565..40849 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
SBBS512_RS00625 41522..42672 + 1151 WP_094103878.1 IS3 family transposase -
SBBS512_RS00630 42706..44244 - 1539 WP_000119266.1 IS66-like element ISEc22 family transposase -
SBBS512_RS00635 44293..44640 - 348 WP_000612625.1 IS66 family insertion sequence element accessory protein TnpB -
SBBS512_RS00640 44637..45017 - 381 WP_001333468.1 IS66 family insertion sequence hypothetical protein -
SBBS512_RS32675 45149..45275 - 127 Protein_59 IS110 family transposase -
SBBS512_RS32680 45406..45510 + 105 Protein_60 DUF1472 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2 / ospD3/senA / ospC1 / senB / ipaH7.8 / ipaH4.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA 1..210919 210919
- inside IScluster/Tn - ospI / ipaH1.4 / ospE2 / ospD3/senA / ospC1 35578..70474 34896


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T22861 WP_000501969.1 NC_010660:c40577-40092 [Shigella boydii CDC 3083-94]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T22861 NC_010660:c40577-40092 [Shigella boydii CDC 3083-94]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT22861 WP_011114751.1 NC_010660:c40849-40565 [Shigella boydii CDC 3083-94]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT22861 NC_010660:c40849-40565 [Shigella boydii CDC 3083-94]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References