Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2526725..2527535 Replicon chromosome
Accession NZ_CP089765
Organism Klebsiella pneumoniae strain HKC2

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LVO53_RS12270 Protein ID WP_004178461.1
Coordinates 2527002..2527535 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LVO53_RS12265 Protein ID WP_002887278.1
Coordinates 2526725..2526991 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO53_RS12245 (2522597) 2522597..2522941 - 345 WP_004197273.1 cation efflux system protein CusF -
LVO53_RS12250 (2522959) 2522959..2524344 - 1386 WP_004178457.1 efflux transporter outer membrane subunit -
LVO53_RS12255 (2524516) 2524516..2525199 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
LVO53_RS12260 (2525189) 2525189..2526622 + 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
LVO53_RS12265 (2526725) 2526725..2526991 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LVO53_RS12270 (2527002) 2527002..2527535 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LVO53_RS12275 (2527583) 2527583..2528704 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T228607 WP_004178461.1 NZ_CP089765:2527002-2527535 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T228607 NZ_CP124326:2963282-2963560 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT228607 WP_002887278.1 NZ_CP089765:2526725-2526991 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT228607 NZ_CP124326:2963089-2963280 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References