Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1935856..1936481 Replicon chromosome
Accession NZ_CP089765
Organism Klebsiella pneumoniae strain HKC2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LVO53_RS09530 Protein ID WP_002882817.1
Coordinates 1936098..1936481 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LVO53_RS09525 Protein ID WP_004150355.1
Coordinates 1935856..1936098 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO53_RS09500 (1931847) 1931847..1932446 + 600 WP_004151865.1 glucose-1-phosphatase -
LVO53_RS09505 (1932440) 1932440..1933300 + 861 WP_004146232.1 virulence factor BrkB family protein -
LVO53_RS09510 (1933297) 1933297..1933734 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LVO53_RS09515 (1933779) 1933779..1934720 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LVO53_RS09520 (1934734) 1934734..1935651 - 918 WP_004181612.1 alpha/beta hydrolase -
LVO53_RS09525 (1935856) 1935856..1936098 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LVO53_RS09530 (1936098) 1936098..1936481 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LVO53_RS09535 (1936655) 1936655..1937584 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LVO53_RS09540 (1937581) 1937581..1938216 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LVO53_RS09545 (1938213) 1938213..1939115 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T228604 WP_002882817.1 NZ_CP089765:1936098-1936481 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T228604 NZ_CP124326:2266988-2267090 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT228604 WP_004150355.1 NZ_CP089765:1935856-1936098 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT228604 NZ_CP124326:c2267128-2266860 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References