Detailed information of TA system TA228602

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1067806..1068463
• Replicon: chromosome
• Accession: NZ_CP089765
• Organism: Klebsiella pneumoniae strain HKC2

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: LVO53_RS05260
• Protein ID: WP_004181233.1
• Coordinates: 1067806..1068216 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: LVO53_RS05265
• Protein ID: WP_002916312.1
• Coordinates: 1068197..1068463 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVO53_RS05240 (1063806)	1063806..1065539	-	1734	WP_277709752.1	single-stranded-DNA-specific exonuclease RecJ	-
LVO53_RS05245 (1065545)	1065545..1066258	-	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
LVO53_RS05250 (1066281)	1066281..1067177	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
LVO53_RS05255 (1067278)	1067278..1067799	+	522	WP_004144730.1	flavodoxin FldB	-
LVO53_RS05260 (1067806)	1067806..1068216	-	411	WP_004181233.1	protein YgfX	Toxin
LVO53_RS05265 (1068197)	1068197..1068463	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
LVO53_RS05270 (1068709)	1068709..1069692	+	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
LVO53_RS05275 (1069843)	1069843..1070502	-	660	WP_002916317.1	hemolysin III family protein	-
LVO53_RS05280 (1070666)	1070666..1070977	-	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
LVO53_RS05285 (1071027)	1071027..1071755	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
LVO53_RS05290 (1071874)	1071874..1073307	+	1434	WP_004181234.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T228602 WP_004181233.1 NZ_CP089765:c1068216-1067806 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T228602 NZ_CP124326:2010876-2011130 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT228602 WP_002916312.1 NZ_CP089765:c1068463-1068197 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT228602 NZ_CP124326:2010628-2010879 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41