Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 56747..57390 Replicon plasmid unnamed1
Accession NZ_CP089763
Organism Klebsiella pneumoniae strain HKC1

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LVO51_RS27945 Protein ID WP_001044770.1
Coordinates 56747..57163 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LVO51_RS27950 Protein ID WP_001261282.1
Coordinates 57160..57390 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO51_RS27925 (52850) 52850..53122 - 273 Protein_71 transposase -
LVO51_RS27935 (54104) 54104..55126 - 1023 WP_000361404.1 helicase UvrD -
LVO51_RS27940 (55111) 55111..56673 - 1563 WP_004206609.1 AAA family ATPase -
LVO51_RS27945 (56747) 56747..57163 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LVO51_RS27950 (57160) 57160..57390 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVO51_RS27955 (57347) 57347..57808 + 462 WP_014343465.1 hypothetical protein -
LVO51_RS27960 (57969) 57969..58913 + 945 WP_011977810.1 hypothetical protein -
LVO51_RS27965 (58950) 58950..59342 + 393 WP_011977811.1 hypothetical protein -
LVO51_RS27970 (59400) 59400..59921 + 522 WP_013214008.1 hypothetical protein -
LVO51_RS27975 (59967) 59967..60170 + 204 WP_011977813.1 hypothetical protein -
LVO51_RS27980 (60200) 60200..61204 + 1005 WP_011977814.1 hypothetical protein -
LVO51_RS27985 (61388) 61388..62167 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / blaTEM-1B / rmtB / blaKPC-2 - 1..135709 135709


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T228599 WP_001044770.1 NZ_CP089763:c57163-56747 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T228599 NZ_CP124326:c825571-825194 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT228599 WP_001261282.1 NZ_CP089763:c57390-57160 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT228599 NZ_CP124326:c826028-825648 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References