Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4633175..4633800 Replicon chromosome
Accession NZ_CP089762
Organism Klebsiella pneumoniae strain HKC1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LVO51_RS22955 Protein ID WP_002882817.1
Coordinates 4633175..4633558 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LVO51_RS22960 Protein ID WP_004150355.1
Coordinates 4633558..4633800 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO51_RS22940 (LVO51_22905) 4630541..4631443 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LVO51_RS22945 (LVO51_22910) 4631440..4632075 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LVO51_RS22950 (LVO51_22915) 4632072..4633001 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LVO51_RS22955 (LVO51_22920) 4633175..4633558 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LVO51_RS22960 (LVO51_22925) 4633558..4633800 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LVO51_RS22965 (LVO51_22930) 4634005..4634922 + 918 WP_002882812.1 alpha/beta hydrolase -
LVO51_RS22970 (LVO51_22935) 4634936..4635877 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LVO51_RS22975 (LVO51_22940) 4635922..4636359 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LVO51_RS22980 (LVO51_22945) 4636356..4637216 - 861 WP_277708870.1 virulence factor BrkB family protein -
LVO51_RS22985 (LVO51_22950) 4637210..4637809 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T228592 WP_002882817.1 NZ_CP089762:c4633558-4633175 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T228592 NZ_CP124324:c4550054-4549881 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT228592 WP_004150355.1 NZ_CP089762:c4633800-4633558 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT228592 NZ_CP124324:c4550378-4550082 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References