Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3429428..3430047 Replicon chromosome
Accession NZ_CP089762
Organism Klebsiella pneumoniae strain HKC1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LVO51_RS17165 Protein ID WP_002892050.1
Coordinates 3429829..3430047 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LVO51_RS17160 Protein ID WP_002892066.1
Coordinates 3429428..3429802 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVO51_RS17150 (LVO51_17120) 3424580..3425773 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LVO51_RS17155 (LVO51_17125) 3425796..3428942 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LVO51_RS17160 (LVO51_17130) 3429428..3429802 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LVO51_RS17165 (LVO51_17135) 3429829..3430047 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LVO51_RS17170 (LVO51_17140) 3430206..3430772 + 567 WP_002892030.1 maltose O-acetyltransferase -
LVO51_RS17175 (LVO51_17145) 3430744..3430884 - 141 WP_004147370.1 hypothetical protein -
LVO51_RS17180 (LVO51_17150) 3430905..3431375 + 471 WP_002892026.1 YlaC family protein -
LVO51_RS17185 (LVO51_17155) 3431350..3432801 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LVO51_RS17190 (LVO51_17160) 3432902..3433600 + 699 WP_002892021.1 GNAT family protein -
LVO51_RS17195 (LVO51_17165) 3433597..3433737 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LVO51_RS17200 (LVO51_17170) 3433737..3434000 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T228588 WP_002892050.1 NZ_CP089762:3429829-3430047 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T228588 NZ_CP124324:c4046139-4045825 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT228588 WP_002892066.1 NZ_CP089762:3429428-3429802 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT228588 NZ_CP124324:c4046375-4046142 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References