Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4916636..4917278 Replicon chromosome
Accession NZ_CP089743
Organism Bacillus paranthracis strain SHOU-BC01

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag LU294_RS25405 Protein ID WP_000635963.1
Coordinates 4916636..4916986 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LU294_RS25410 Protein ID WP_000004570.1
Coordinates 4916991..4917278 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU294_RS25390 (LU294_25390) 4913572..4914030 - 459 WP_109631580.1 SprT family protein -
LU294_RS25395 (LU294_25395) 4914226..4914342 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LU294_RS25400 (LU294_25400) 4914400..4916568 - 2169 WP_142423024.1 Tex family protein -
LU294_RS25405 (LU294_25405) 4916636..4916986 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LU294_RS25410 (LU294_25410) 4916991..4917278 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LU294_RS25415 (LU294_25415) 4917578..4918747 - 1170 WP_000390599.1 alanine racemase -
LU294_RS25420 (LU294_25420) 4918866..4919816 - 951 WP_025991724.1 outer membrane lipoprotein carrier protein LolA -
LU294_RS25425 (LU294_25425) 4919973..4920332 - 360 WP_000635037.1 holo-ACP synthase -
LU294_RS25430 (LU294_25430) 4920425..4920997 + 573 WP_000906924.1 rhomboid family intramembrane serine protease -
LU294_RS25435 (LU294_25435) 4920990..4921952 - 963 WP_109631574.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T228571 WP_000635963.1 NZ_CP089743:c4916986-4916636 [Bacillus paranthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T228571 NZ_CP124324:188058-188165 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT228571 WP_000004570.1 NZ_CP089743:c4917278-4916991 [Bacillus paranthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT228571 NZ_CP124324:c188009-187944 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References