Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2770762..2771737 Replicon chromosome
Accession NZ_CP089743
Organism Bacillus paranthracis strain SHOU-BC01

Toxin (Protein)


Gene name spoIISA Uniprot ID C2MKV2
Locus tag LU294_RS14455 Protein ID WP_001997496.1
Coordinates 2771000..2771737 (-) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A402GGJ7
Locus tag LU294_RS14450 Protein ID WP_000594783.1
Coordinates 2770762..2770887 (-) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU294_RS14405 (LU294_14405) 2765762..2766295 - 534 WP_000438330.1 sigma-70 family RNA polymerase sigma factor -
LU294_RS14410 (LU294_14410) 2766518..2767111 - 594 WP_000265898.1 SGNH/GDSL hydrolase family protein -
LU294_RS14415 (LU294_14415) 2767254..2767613 + 360 WP_109629521.1 DUF5065 family protein -
LU294_RS14420 (LU294_14420) 2767702..2769171 - 1470 WP_109629518.1 beta-Ala-His dipeptidase -
LU294_RS14425 (LU294_14425) 2769315..2769491 - 177 WP_000808045.1 stage II sporulation protein SB -
LU294_RS14430 (LU294_14430) 2769568..2769651 - 84 Protein_2854 D-alanyl-D-alanine carboxypeptidase -
LU294_RS14435 (LU294_14435) 2769825..2770049 + 225 WP_142422979.1 WXG100 family type VII secretion target -
LU294_RS14440 (LU294_14440) 2770191..2770466 + 276 WP_001041552.1 hypothetical protein -
LU294_RS14445 (LU294_14445) 2770510..2770686 - 177 WP_000819051.1 stage II sporulation protein SB -
LU294_RS14450 (LU294_14450) 2770762..2770887 - 126 WP_000594783.1 hypothetical protein Antitoxin
LU294_RS14455 (LU294_14455) 2771000..2771737 - 738 WP_001997496.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
LU294_RS14460 (LU294_14460) 2771926..2772663 - 738 WP_000594125.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
LU294_RS14465 (LU294_14465) 2772831..2773313 - 483 WP_000191884.1 MarR family winged helix-turn-helix transcriptional regulator -
LU294_RS14470 (LU294_14470) 2773410..2775095 + 1686 WP_001997495.1 alpha-keto acid decarboxylase family protein -
LU294_RS14475 (LU294_14475) 2775217..2775999 - 783 WP_232685338.1 class I SAM-dependent methyltransferase -
LU294_RS14480 (LU294_14480) 2776284..2776691 - 408 WP_000072475.1 VOC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28377.08 Da        Isoelectric Point: 8.2672

>T228570 WP_001997496.1 NZ_CP089743:c2771737-2771000 [Bacillus paranthracis]
VISNIRIGLFILAIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTMIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T228570 NZ_CP124323:c142271-141966 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4879.29 Da        Isoelectric Point: 3.9798

>AT228570 WP_000594783.1 NZ_CP089743:c2770887-2770762 [Bacillus paranthracis]
VIKNSDNDFFKEDNEDTTDFSLIKGDTMQQVEELEEELKRK

Download         Length: 126 bp

>AT228570 NZ_CP124323:c142491-142273 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1T2Q3U8

Antitoxin

Source ID Structure
AlphaFold DB A0A402GGJ7

References