Detailed information of TA system TA228563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2432783..2434281
• Replicon: chromosome
• Accession: NZ_CP089742
• Organism: Melaminivora jejuensis strain KACC 19629

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: LVC68_RS11290
• Protein ID: WP_209421530.1
• Coordinates: 2432783..2433685 (-)
• Length: 301 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: LVC68_RS11295
• Protein ID: WP_106684098.1
• Coordinates: 2433682..2434281 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVC68_RS11290 (LVC68_11290)	2432783..2433685	-	903	WP_209421530.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LVC68_RS11295 (LVC68_11295)	2433682..2434281	-	600	WP_106684098.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
LVC68_RS11300 (LVC68_11300)	2434455..2435009	-	555	WP_209421529.1	hypothetical protein	-
LVC68_RS11305 (LVC68_11305)	2435169..2436494	+	1326	WP_209421528.1	ISL3 family transposase	-
LVC68_RS11310 (LVC68_11310)	2436641..2437900	+	1260	WP_209421527.1	DNA repair exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2407823..2449267	41444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-256)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (6-52)

Sequences

Toxin

Protein

• Length: 301 a.a.
• Molecular weight: 33154.89 Da
• Isoelectric Point: 5.8847

>T228563 WP_209421530.1 NZ_CP089742:c2433685-2432783 [Melaminivora jejuensis]
MSANLSASILARLLTLAKQRDDDYSLLLNRFGMERLLARVSSSPYAERFLLKGALLFALWYDTPHRPTRDADLLGFGPDD
ETNLIATFREVAAMDLDDGIVFDPESVKADAIREDNTYGGTRITLVARIGSARCALQIDVGFGDAVTPGPQTVTYPTLLD
DFQAPTLRVYPVYTVIAEKYQAMVMLGQANSRMKDFFDLTVIARRTELDGATLATAITATFARRQTALPTGRPLALTKQF
SEDPAKLRQWQAFLNKNRIAAASLGDTIALLDDLLWPPTQVAAAHSQAKATWRPDSLRWV

Nucleotide

• Length: 903 bp

>T228563 NZ_CP124322:3631504-3631722 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22332.84 Da
• Isoelectric Point: 9.6695

>AT228563 WP_106684098.1 NZ_CP089742:c2434281-2433682 [Melaminivora jejuensis]
MNQSDYILELARHRRVLRAADVRAQGWSPQLLIRLHQAGKLHRLGRGLYGLPDAEVTEHQTLIEVCQRVPKAVLCLLSAL
QFHEIGTQLPHEVWVALPEGTQAPAPGYPALRITRLRGAAYSDGIQTVAEHGAPIRVYSAAKTVTDCFKFRNKIGLDVAL
EALKDAWRSRKVTMDELGHFAKINRVERVMQPYLEAVTQ

Nucleotide

• Length: 600 bp

>AT228563 NZ_CP124322:3631104-3631478 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG