Detailed information of TA system TA228561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3810673..3811358
• Replicon: chromosome
• Accession: NZ_CP089611
• Organism: Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: LVJ90_RS17920
• Protein ID: WP_003417998.1
• Coordinates: 3810948..3811358 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LVJ90_RS17915
• Protein ID: WP_003912220.1
• Coordinates: 3810673..3810951 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ90_RS17895 (LVJ90_17895)	3806662..3808263	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
LVJ90_RS17900 (LVJ90_17900)	3808280..3808984	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
LVJ90_RS17905 (LVJ90_17905)	3809102..3809668	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
LVJ90_RS17910 (LVJ90_17910)	3809730..3810617	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
LVJ90_RS17915 (LVJ90_17915)	3810673..3810951	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ90_RS17920 (LVJ90_17920)	3810948..3811358	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ90_RS17925 (LVJ90_17925)	3811391..3813127	-	1737	WP_003418002.1	cholesterol oxidase	-
LVJ90_RS17930 (LVJ90_17930)	3813183..3814310	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
LVJ90_RS17935 (LVJ90_17935)	3814330..3815919	-	1590	WP_075917289.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T228561 WP_003417998.1 NZ_CP089611:3810948-3811358 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T228561 NZ_CP124322:2963283-2963561 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT228561 WP_003912220.1 NZ_CP089611:3810673-3810951 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT228561 NZ_CP124322:2963090-2963281 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: G0TIR9