Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3168913..3169600 Replicon chromosome
Accession NZ_CP089611
Organism Mycobacterium tuberculosis strain I0004240-3

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag LVJ90_RS15105 Protein ID WP_003414624.1
Coordinates 3169157..3169600 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag LVJ90_RS15100 Protein ID WP_003414620.1
Coordinates 3168913..3169170 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ90_RS15080 (LVJ90_15080) 3164233..3165087 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
LVJ90_RS15085 (LVJ90_15085) 3165143..3166306 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
LVJ90_RS15090 (LVJ90_15090) 3166323..3167537 - 1215 WP_003899518.1 zinc metalloprotease Rip -
LVJ90_RS15095 (LVJ90_15095) 3167545..3168786 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
LVJ90_RS15100 (LVJ90_15100) 3168913..3169170 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
LVJ90_RS15105 (LVJ90_15105) 3169157..3169600 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ90_RS15110 (LVJ90_15110) 3169680..3170342 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
LVJ90_RS15115 (LVJ90_15115) 3170438..3170629 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
LVJ90_RS15120 (LVJ90_15120) 3170961..3172709 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
LVJ90_RS15125 (LVJ90_15125) 3172805..3173386 + 582 WP_003900589.1 fasciclin domain-containing protein -
LVJ90_RS15130 (LVJ90_15130) 3173486..3173752 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T228556 WP_003414624.1 NZ_CP089611:3169157-3169600 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T228556 NZ_CP124322:2010992-2011246 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT228556 WP_003414620.1 NZ_CP089611:3168913-3169170 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT228556 NZ_CP124322:2010744-2010995 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References